| Blackbanded Sunfish | Enneacanthus | chaetodon | | 7 | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Barking Treefrog | Dryophytes | gratiosus | | 19 | Hylidae | Anura | Amphibia | Animalia |
| Mountain Chorus Frog | Pseudacris | brachyphona | | 17 | Hylidae | Anura | Amphibia | Animalia |
| Eastern Narrow-mouthed Toad | Gastrophryne | carolinensis | | 46 | Microhylidae | Anura | Amphibia | Animalia |
| Eastern Tiger Salamander | Ambystoma | tigrinum | | 56 | Ambystomatidae | Caudata | Amphibia | Animalia |
| Hellbender | Cryptobranchus | alleganiensis | | 16 | Cryptobranchidae | Caudata | Amphibia | Animalia |
| Green Salamander | Aneides | aeneus | | 41 | Plethodontidae | Caudata | Amphibia | Animalia |
| Appalachian Tiger Beetle | Cicindela | ancocisconensis | | 27 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Northern Barrens Tiger Beetle | Cicindela | patruela | | 30 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Splendid Tiger Beetle | Cicindela | splendida | | 32 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Pepper and Salt Skipper | Amblyscirtes | hegon | | 67 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Rare Skipper | Atrytone | bulenta | | 39 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Chermock's Mulberry Wing | Poanes | massasoit | | 0 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Appalachian Grizzled Skipper | Pyrgus | centaureae | | 10 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Great Purple Hairstreak | Atlides | halesus | | 95 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Frosted Elfin | Callophrys | irus | | 65 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Hoary Elfin | Callophrys | polios | | 2 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Bog Copper | Lycaena | epixanthe | | 44 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Edwards' Hairstreak | Satyrium | edwardsii | | 69 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| King's Hairstreak | Satyrium | kingi | | 25 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Harris's Checkerspot | Chlosyne | harrisii | | 39 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Baltimore Checkerspot | Euphydryas | phaeton | | 165 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Atlantis Fritillary | Speyeria | atlantis | | 26 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Olympia Marble | Euchloe | olympia | | 102 | Pieridae | Lepidoptera | Insecta | Animalia |
| St. Croix Snaketail | Ophiogomphus | susbehcha | | 14 | Gomphidae | Odonata | Insecta | Animalia |
| No Common Name | Caecidotea | kenki | | 27 | Asellidae | Isopoda | Malacostraca | Animalia |
| Eastern Small-footed Bat | Myotis | leibii | | 26 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Indiana Bat | Myotis | sodalis | | 20 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Rainbow Snake | Farancia | erytrogramma | | 13 | Colubridae | Squamata | Reptilia | Animalia |
| Mountain Earthsnake | Virginia | valeriae | | 15 | Colubridae | Squamata | Reptilia | Animalia |
| Timber Rattlesnake | Crotalus | horridus | | 180 | Crotalidae | Squamata | Reptilia | Animalia |
| Coal Skink | Plestiodon | anthracinus | | 8 | Scincidae | Squamata | Reptilia | Animalia |
| Spotted Turtle | Clemmys | guttata | | 380 | Emydidae | Testudines | Reptilia | Animalia |
| Wood Turtle | Glyptemys | insculpta | | 196 | Emydidae | Testudines | Reptilia | Animalia |
| Bog Turtle | Glyptemys | muhlenbergii | | 196 | Emydidae | Testudines | Reptilia | Animalia |
| Northern Map Turtle | Graptemys | geographica | | 76 | Emydidae | Testudines | Reptilia | Animalia |
| Spiny Softshell | Apalone | spinifera | | 11 | Trionychidae | Testudines | Reptilia | Animalia |
| Canby's Cowbane | Tiedemannia | canbyi | | 3 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| American Ginseng | Panax | quinquefolius | | 31 | Araliaceae | Apiales | Magnoliopsida | Plantae |
| Atamasco Lily | Zephyranthes | atamasca | | 1 | Amaryllidaceae | Asparagales | Magnoliopsida | Plantae |
| Canby's Mountain-lover | Paxistima | canbyi | | 3 | Celastraceae | Celastrales | Magnoliopsida | Plantae |
| Pygmy Pipes | Monotropsis | odorata | | 34 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Northern Pitcherplant | Sarracenia | purpurea | | 159 | Sarraceniaceae | Ericales | Magnoliopsida | Plantae |
| Sundial Lupine | Lupinus | perennis | | 116 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Fringe-tipped Closed Gentian | Gentiana | andrewsii | | 13 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Striped Gentian | Gentiana | villosa | | 46 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Greater Fringed Gentian | Gentianopsis | crinita | | 80 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Sandplain Gerardia | Agalinis | decemloba | | 20 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Swamp Pink | Helonias | bullata | | 37 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Drooping Trillium | Trillium | flexipes | | 62 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Snow Trillium | Trillium | nivale | | 47 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Dwarf Trillium | Trillium | pusillum | | 51 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Common Grass-pink | Calopogon | tuberosus | | 44 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Spreading Pogonia | Cleistesiopsis | divaricata | | 5 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Early Coralroot | Corallorhiza | trifida | | 5 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Spring Coralroot | Corallorhiza | wisteriana | | 37 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Small White Lady's Slipper | Cypripedium | candidum | | 12 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Long-bracted Orchid | Dactylorhiza | viridis | | 35 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Appalachian Twayblade | Neottia | smallii | | 26 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| White-fringed Orchid | Platanthera | blephariglottis | | 41 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Yellow-fringed Orchid | Platanthera | ciliaris | | 68 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Crested Yellow Orchid | Platanthera | cristata | | 81 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Greater Purple-fringed Orchid | Platanthera | grandiflora | | 26 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Purple Fringeless Orchid | Platanthera | peramoena | | 139 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Shriver's Orchid | Platanthera | shriveri | | 12 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Shining Lady's Tresses | Spiranthes | lucida | | 13 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Yellow Nodding Lady's Tresses | Spiranthes | ochroleuca | | 47 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Nodding Pogonia | Triphora | trianthophora | | 14 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Harper's Fimbry | Fimbristylis | perpusilla | | 40 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Tall Larkspur | Delphinium | exaltatum | | 14 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Goldenseal | Hydrastis | canadensis | | 40 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |