| American Toad | Anaxyrus | americanus americanus | | 4 | Bufonidae | Anura | Amphibia | Animalia |
| Fowler's Toad | Anaxyrus | fowleri | | 2 | Bufonidae | Anura | Amphibia | Animalia |
| Eastern Cricket Frog | Acris | crepitans crepitans | | 1 | Hylidae | Anura | Amphibia | Animalia |
| Gray Treefrog | Hyla | versicolor | | 3 | Hylidae | Anura | Amphibia | Animalia |
| Spring Peeper | Pseudacris | crucifer crucifer | | 8 | Hylidae | Anura | Amphibia | Animalia |
| American Bullfrog | Lithobates | catesbeianus | | 1 | Ranidae | Anura | Amphibia | Animalia |
| Green Frog | Lithobates | clamitans | | 3 | Ranidae | Anura | Amphibia | Animalia |
| Pickerel Frog | Lithobates | palustris | | 6 | Ranidae | Anura | Amphibia | Animalia |
| Southern Leopard Frog | Lithobates | sphenocephalus utricularius | | 8 | Ranidae | Anura | Amphibia | Animalia |
| Eastern Spadefoot | Scaphiopus | holbrookii | | 1 | Scaphiopodidae | Anura | Amphibia | Animalia |
| Spotted Salamander | Ambystoma | maculatum | | 2 | Ambystomatidae | Caudata | Amphibia | Animalia |
| Marbled Salamander | Ambystoma | opacum | | 4 | Ambystomatidae | Caudata | Amphibia | Animalia |
| Eastern Red-backed Salamander | Plethodon | cinereus | | 2 | Plethodontidae | Caudata | Amphibia | Animalia |
| Eastern Newt | Notophthalmus | viridescens | | 1 | Salamandridae | Caudata | Amphibia | Animalia |
| Arrowhead Spider | Verrucosa | arenata | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| No Common Name | Dolomedes | tenebrosus | | 1 | Pisauridae | Araneae | Arachnida | Animalia |
| Leopard Slug | Limax | maximus | | 1 | Limacidae | Stylommatophora | Gastropoda | Animalia |
| Six-spotted Tiger Beetle | Cicindela | sexguttata | | 1 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Eyed Click Beetle | Alaus | oculatus | | 1 | Elateridae | Coleoptera | Insecta | Animalia |
| Reddish-brown Stag Beetle | Lucanus | capreolus | | 1 | Lucanidae | Coleoptera | Insecta | Animalia |
| Patent Leather Beetle | Odontotaenius | disjunctus | | 1 | Passalidae | Coleoptera | Insecta | Animalia |
| Red-footed Cannibalfly | Promachus | rufipes | | 2 | Asilidae | Diptera | Insecta | Animalia |
| Northern Triorla | Triorla | interrupta | | 1 | Asilidae | Diptera | Insecta | Animalia |
| Western Leaf-footed Bug | Leptoglossus | clypealis | | 1 | Coreidae | Hemiptera | Insecta | Animalia |
| No Common Name | Cyrpoptus | belfragei | | 1 | Fulgoridae | Hemiptera | Insecta | Animalia |
| Spotted Lanternfly | Lycorma | delicatula | | 4 | Fulgoridae | Hemiptera | Insecta | Animalia |
| Florida Predatory Stink Bug | Euthyrhynchus | floridanus | | 2 | Pentatomidae | Hemiptera | Insecta | Animalia |
| Four-toothed Mason Wasp | Monobia | quadridens | | 1 | Vespidae | Hymenoptera | Insecta | Animalia |
| Giant Leopard Moth | Hypercompe | scribonia | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Greater Grapevine Looper Moth | Eulithis | gracilineata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Least Skipper | Ancyloxypha | numitor | | 3 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Silver-spotted Skipper | Epargyreus | clarus | | 3 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Zabulon Skipper | Lon | zabulon | | 2 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Broad-winged Skipper | Poanes | viator | | 5 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Peck's Skipper | Polites | peckius | | 1 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Red-banded Hairstreak | Calycopis | cecrops | | 1 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Eastern Tailed-Blue | Cupido | comyntas orientalis | | 2 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Gray Hairstreak | Strymon | melinus | | 1 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| American Dagger Moth | Acronicta | americana | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Tawny Emperor | Asterocampa | clyton | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Monarch | Danaus | plexippus | | 3 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Northern Pearly-eye | Lethe | anthedon | | 2 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Appalachian Brown | Lethe | appalachia | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Viceroy | Limenitis | archippus | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Red-spotted Purple | Limenitis | arthemis astyanax | | 4 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Mourning Cloak | Nymphalis | antiopa | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Pearl Crescent | Phyciodes | tharos | | 3 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Red Admiral | Vanessa | atalanta | | 2 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Zebra Swallowtail | Eurytides | marcellus | | 3 | Papilionidae | Lepidoptera | Insecta | Animalia |
| Eastern Tiger Swallowtail | Pterourus | glaucus | | 1 | Papilionidae | Lepidoptera | Insecta | Animalia |
| Sleepy Orange | Abaeis | nicippe | | 1 | Pieridae | Lepidoptera | Insecta | Animalia |
| Cloudless Sulphur | Phoebis | sennae | | 1 | Pieridae | Lepidoptera | Insecta | Animalia |
| Luna Moth | Actias | luna | | 9 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Io Moth | Automeris | io | | 1 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Lilypad Forktail | Ischnura | kellicotti | | 1 | Coenagrionidae | Odonata | Insecta | Animalia |
| Common Pondhawk | Erythemis | simplicicollis | | 2 | Libellulidae | Odonata | Insecta | Animalia |
| Spangled Skimmer | Libellula | cyanea | | 1 | Libellulidae | Odonata | Insecta | Animalia |
| Great Blue Skimmer | Libellula | vibrans | | 1 | Libellulidae | Odonata | Insecta | Animalia |
| Common Whitetail | Plathemis | lydia | | 3 | Libellulidae | Odonata | Insecta | Animalia |
| Carolina Saddlebags | Tramea | carolina | | 1 | Libellulidae | Odonata | Insecta | Animalia |
| Northern Mole Cricket | Neocurtilla | hexadactyla | | 1 | Gryllotalpidae | Orthoptera | Insecta | Animalia |
| Protean Shieldback | Atlanticus | testaceus | | 1 | Tettigoniidae | Orthoptera | Insecta | Animalia |
| Delmarva Fox Squirrel | Sciurus | niger cinereus | | 1 | Sciuridae | Rodentia | Mammalia | Animalia |
| Northern Short-tailed Shrew | Blarina | brevicauda | | 2 | Soricidae | Soricomorpha | Mammalia | Animalia |
| Eastern Wormsnake | Carphophis | amoenus amoenus | | 3 | Colubridae | Squamata | Reptilia | Animalia |
| Northern Black Racer | Coluber | constrictor constrictor | | 11 | Colubridae | Squamata | Reptilia | Animalia |
| Ring-necked Snake | Diadophis | punctatus | | 2 | Colubridae | Squamata | Reptilia | Animalia |
| Northern Watersnake | Nerodia | sipedon sipedon | | 3 | Colubridae | Squamata | Reptilia | Animalia |
| Rough Greensnake | Opheodrys | aestivus | | 7 | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Ratsnake | Pantherophis | alleghaniensis | | 14 | Colubridae | Squamata | Reptilia | Animalia |
| Dekay's Brownsnake | Storeria | dekayi | | 41 | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Ribbonsnake | Thamnophis | saurita | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Common Gartersnake | Thamnophis | sirtalis | | 27 | Colubridae | Squamata | Reptilia | Animalia |
| Common Five-lined Skink | Plestiodon | fasciatus | | 4 | Scincidae | Squamata | Reptilia | Animalia |
| Snapping Turtle | Chelydra | serpentina | | 3 | Chelydridae | Testudines | Reptilia | Animalia |
| Eastern Painted Turtle | Chrysemys | picta picta | | 1 | Emydidae | Testudines | Reptilia | Animalia |
| Northern Red-bellied Turtle | Pseudemys | rubriventris | | 1 | Emydidae | Testudines | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 6 | Emydidae | Testudines | Reptilia | Animalia |
| Eastern Mud Turtle | Kinosternon | subrubrum | | 1 | Kinosternidae | Testudines | Reptilia | Animalia |
| Eastern Musk Turtle | Sternotherus | odoratus | | 1 | Kinosternidae | Testudines | Reptilia | Animalia |
| Coral-pink Merulius | Byssomerulius | incarnatus | | 1 | Meruliaceae | Polyporales | Agaricomycetes | Fungi |
| Cardinal Flower | Lobelia | cardinalis | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Spotted Wintergreen | Chimaphila | maculata | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Pinesap | Monotropa | hypopitys | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| False Indigo Bush | Amorpha | fruticosa | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |