| Pearl Dace | Margariscus | margarita | | 20 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Flier | Centrarchus | macropterus | | 34 | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Glassy Darter | Etheostoma | vitreum | | 54 | Percidae | Perciformes | Actinopterygii | Animalia |
| Chesapeake Logperch | Percina | bimaculata | | 32 | Percidae | Perciformes | Actinopterygii | Animalia |
| Logperch | Percina | caprodes | | 0 | Percidae | Perciformes | Actinopterygii | Animalia |
| Least Tern | Sternula | antillarum | | 387 | Laridae | Charadriiformes | Aves | Animalia |
| Red Knot | Calidris | canutus | | 96 | Scolopacidae | Charadriiformes | Aves | Animalia |
| Nashville Warbler | Leiothlypis | ruficapilla | | 254 | Parulidae | Passeriformes | Aves | Animalia |
| American Bittern | Botaurus | lentiginosus | | 272 | Ardeidae | Pelecaniformes | Aves | Animalia |
| American Brook Lamprey | Lethenteron | appendix | | 28 | Petromyzontidae | Petromyzontiformes | Cephalaspidomorphi | Animalia |
| Rare Skipper | Atrytone | bulenta | | 39 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Great Purple Hairstreak | Atlides | halesus | | 95 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Harris's Checkerspot | Chlosyne | harrisii | | 39 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Atlantis Fritillary | Speyeria | atlantis brittanae | | 25 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Northern Metalmark | Calephelis | borealis | | 202 | Riodinidae | Lepidoptera | Insecta | Animalia |
| Superb Jewelwing | Calopteryx | amata | | 44 | Calopterygidae | Odonata | Insecta | Animalia |
| Selys' Sundragon | Helocordulia | selysii | | 54 | Corduliidae | Odonata | Insecta | Animalia |
| Northern Myotis | Myotis | septentrionalis | | 12 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Loggerhead Sea Turtle | Caretta | caretta | | 42 | Cheloniidae | Testudines | Reptilia | Animalia |
| Green Sea Turtle | Chelonia | mydas | | 4 | Cheloniidae | Testudines | Reptilia | Animalia |
| Bog Turtle | Glyptemys | muhlenbergii | | 196 | Emydidae | Testudines | Reptilia | Animalia |
| Engelmann's Arrowhead | Sagittaria | engelmanniana | | 32 | Alismataceae | Alismatales | Magnoliopsida | Plantae |
| Mountain Pimpernel | Taenidia | montana | | 15 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Single-head Pussytoes | Antennaria | solitaria | | 83 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Hairy Elephant's-foot | Elephantopus | tomentosus | | 11 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Justiceweed | Eupatorium | leucolepis | | 34 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Few-leaved Sunflower | Helianthus | occidentalis ssp. occidentalis | | 5 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Camphor Fleabane | Pluchea | camphorata | | 54 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Pink Bogbutton | Sclerolepis | uniflora | | 29 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Racemose Goldenrod | Solidago | racemosa | | 25 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Showy Goldenrod | Solidago | speciosa var. speciosa | | 21 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Mapleleaf Goosefoot | Chenopodiastrum | simplex | | 16 | Amaranthaceae | Caryophyllales | Magnoliopsida | Plantae |
| Michaux's Stitchwort | Minuartia | michauxii var. michauxii | | 16 | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Quill Fameflower | Phemeranthus | teretifolius | | 109 | Montiaceae | Caryophyllales | Magnoliopsida | Plantae |
| Common Snowberry | Symphoricarpos | albus | | 6 | Caprifoliaceae | Dipsacales | Magnoliopsida | Plantae |
| Leatherleaf | Chamaedaphne | calyculata | | 31 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Small Cranberry | Vaccinium | oxycoccos | | 18 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Northern Pitcherplant | Sarracenia | purpurea | | 159 | Sarraceniaceae | Ericales | Magnoliopsida | Plantae |
| Dwarf Pipewort | Eriocaulon | parkeri | | 61 | Eriocaulaceae | Eriocaulales | Magnoliopsida | Plantae |
| Ozark Milkvetch | Astragalus | distortus | | 28 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Blue Wild indigo | Baptisia | australis | | 61 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Sundial Lupine | Lupinus | perennis ssp. perennis | | 114 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Hairy Snoutbean | Rhynchosia | tomentosa var. tomentosa | | 21 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Spiked Hoarypea | Tephrosia | spicata | | 52 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Kate's Mountain Clover | Trifolium | virginicum | | 165 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Shumard Oak | Quercus | shumardii | | 24 | Fagaceae | Fagales | Magnoliopsida | Plantae |
| Carolina Milkvine | Matelea | carolinensis | | 68 | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Fringe-tipped Closed Gentian | Gentiana | andrewsii var. andrewsii | | 13 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Large-leaved Waterleaf | Hydrophyllum | macrophyllum | | 7 | Boraginaceae | Lamiales | Magnoliopsida | Plantae |
| Coville's Phacelia | Phacelia | covillei | | 75 | Boraginaceae | Lamiales | Magnoliopsida | Plantae |
| Purple Giant Hyssop | Agastache | scrophulariifolia | | 15 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Whorled Mountain-mint | Pycnanthemum | verticillatum | | 22 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Small Skullcap | Scutellaria | leonardii | | 23 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Veiny Skullcap | Scutellaria | nervosa | | 38 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Red Turtlehead | Chelone | obliqua var. obliqua | | 52 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Bluebead | Clintonia | borealis | | 40 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| White Trout Lily | Erythronium | albidum | | 143 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Twistedstalk | Streptopus | lanceolatus var. lanceolatus | | 34 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Eastern Featherbells | Stenanthium | gramineum | | 32 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Dwarf Trillium | Trillium | pusillum var. virginianum | | 51 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Bamboo Vine | Smilax | pseudochina | | 35 | Smilacaceae | Liliales | Magnoliopsida | Plantae |
| Sandplain Flax | Linum | intercursum | | 34 | Linaceae | Linales | Magnoliopsida | Plantae |
| Southern Bayberry | Morella | caroliniensis | | 12 | Myricaceae | Myricales | Magnoliopsida | Plantae |
| Eastern Leatherwood | Dirca | palustris | | 80 | Thymelaeaceae | Myrtales | Magnoliopsida | Plantae |
| White-fringed Orchid | Platanthera | blephariglottis var. blephariglottis | | 41 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Yellow-fringed Orchid | Platanthera | ciliaris | | 68 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Greater Purple-fringed Orchid | Platanthera | grandiflora | | 26 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Purple Fringeless Orchid | Platanthera | peramoena | | 139 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Allegheny Vine | Adlumia | fungosa | | 42 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Wild Bleedinghearts | Dicentra | eximia | | 62 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Pipevine | Isotrema | macrophyllum | | 22 | Aristolochiaceae | Piperales | Magnoliopsida | Plantae |
| Buxbaum's Sedge | Carex | buxbaumii | | 21 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Blister Sedge | Carex | vesicaria | | 7 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Velvet Sedge | Carex | vestita | | 16 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Mid-Atlantic Beakrush | Rhynchospora | mesoatlantica | | 6 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Long-beaked Beaksedge | Rhynchospora | scirpoides | | 11 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Torrey's Beaksedge | Rhynchospora | torreyana | | 13 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Wand-like Threeawn | Aristida | virgata | | 3 | Poaceae | Poales | Magnoliopsida | Plantae |
| Woolly Witchgrass | Dichanthelium | scabriusculum | | 6 | Poaceae | Poales | Magnoliopsida | Plantae |
| Threeflower Melicgrass | Melica | nitens | | 4 | Poaceae | Poales | Magnoliopsida | Plantae |
| Roughleaf Ricegrass | Oryzopsis | asperifolia | | 3 | Poaceae | Poales | Magnoliopsida | Plantae |
| Mudbank Crowngrass | Paspalum | dissectum | | 7 | Poaceae | Poales | Magnoliopsida | Plantae |
| Horsetail Paspalum | Paspalum | fluitans | | 24 | Poaceae | Poales | Magnoliopsida | Plantae |
| Swamp Wedgescale | Sphenopholis | pensylvanica | | 21 | Poaceae | Poales | Magnoliopsida | Plantae |
| Cross-leaved Milkwort | Polygala | cruciata var. cruciata | | 19 | Polygalaceae | Polygalales | Magnoliopsida | Plantae |
| Racemed Milkwort | Polygala | polygama | | 36 | Polygalaceae | Polygalales | Magnoliopsida | Plantae |
| Seneca Snakeroot | Polygala | senega | | 59 | Polygalaceae | Polygalales | Magnoliopsida | Plantae |
| American Featherfoil | Hottonia | inflata | | 37 | Primulaceae | Primulales | Magnoliopsida | Plantae |
| Lowland Yellow Loosestrife | Lysimachia | hybrida | | 16 | Primulaceae | Primulales | Magnoliopsida | Plantae |
| Goldenseal | Hydrastis | canadensis | | 40 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Low Serviceberry | Amelanchier | humilis | | 2 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Nantucket Serviceberry | Amelanchier | nantucketensis | | 6 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Allegheny Plum | Prunus | alleghaniensis | | 35 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Canada Burnet | Sanguisorba | canadensis | | 70 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Eastern Purple Bladderwort | Utricularia | purpurea | | 15 | Lentibulariaceae | Scrophulariales | Magnoliopsida | Plantae |
| Appalachian Jacob's Ladder | Polemonium | vanbruntiae | | 7 | Polemoniaceae | Solanales | Magnoliopsida | Plantae |
| Coppery St. Johnswort | Hypericum | denticulatum var. denticulatum | | 16 | Hypericaceae | Theales | Magnoliopsida | Plantae |
| Northern White Cedar | Thuja | occidentalis | | 48 | Cupressaceae | Pinales | Pinopsida | Plantae |
| American Yew | Taxus | canadensis | | 23 | Taxaceae | Pinales | Pinopsida | Plantae |
| Bradley's Spleenwort | Asplenium | bradleyi | | 13 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Glade Fern | Diplaziopsis | pycnocarpa | | 57 | Diplaziopsidaceae | Polypodiales | Polypodiopsida | Plantae |
| Bog Fern | Thelypteris | simulata | | 32 | Thelypteridaceae | Polypodiales | Polypodiopsida | Plantae |
| Rusty Woodsia | Woodsia | ilvensis | | 14 | Woodsiaceae | Polypodiales | Polypodiopsida | Plantae |
| American Climbing Fern | Lygodium | palmatum | | 130 | Lygodiaceae | Schizaeales | Polypodiopsida | Plantae |