| Blacknose Dace | Rhinichthys | atratulus | | 1 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Green Frog | Lithobates | clamitans | | 1 | Ranidae | Anura | Amphibia | Animalia |
| Northern Cardinal | Cardinalis | cardinalis | | 2 | Cardinalidae | Passeriformes | Aves | Animalia |
| Black-capped Chickadee | Poecile | atricapillus | | 1 | Paridae | Passeriformes | Aves | Animalia |
| Green Heron | Butorides | virescens | | 1 | Ardeidae | Pelecaniformes | Aves | Animalia |
| Hermit Flower Beetle | Osmoderma | eremicola | | 1 | Scarabaeidae | Coleoptera | Insecta | Animalia |
| No Common Name | Leptoglossus | oppositus | | 1 | Coreidae | Hemiptera | Insecta | Animalia |
| No Common Name | Ormenoides | venusta | | 1 | Flatidae | Hemiptera | Insecta | Animalia |
| Columbine Sawfly | Pristiphora | rufipes | | 1 | Tenthredinidae | Hymenoptera | Insecta | Animalia |
| Hickory Tussock Moth | Lophocampa | caryae | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Gray Furcula Moth | Furcula | cinerea | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Angulose Prominent Moth | Peridea | angulosa | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Great Spangled Fritillary | Argynnis | cybele | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Eastern Ratsnake | Pantherophis | alleghaniensis | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Wood Turtle | Glyptemys | insculpta | | 1 | Emydidae | Testudines | Reptilia | Animalia |
| Luminescent Panellus | Panellus | stipticus | | 2 | Mycenaceae | Agaricales | Agaricomycetes | Fungi |
| Mock Oyster | Phyllotopsis | nidulans | | 1 | Phyllotopsidaceae | Agaricales | Agaricomycetes | Fungi |
| Velvet Shank | Flammulina | velutipes | | 1 | Physalacriaceae | Agaricales | Agaricomycetes | Fungi |
| Fire Cap Marasmius | Rhizomarasmius | pyrrhocephalus | | 1 | Physalacriaceae | Agaricales | Agaricomycetes | Fungi |
| Common Split Gill | Schizophyllum | commune | | 1 | Schizophyllaceae | Agaricales | Agaricomycetes | Fungi |
| Stalked Puffball-in-Aspic | Calostoma | cinnabarinum | | 1 | Sclerodermataceae | Boletales | Agaricomycetes | Fungi |
| Collared Calostoma | Calostoma | lutescens | | 2 | Sclerodermataceae | Boletales | Agaricomycetes | Fungi |
| Veiled Polypore | Cryptoporus | volvatus | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Tinder Polypore | Fomes | fomentarius | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Hexagonal-pored Polypore | Neofavolus | alveolaris | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Violet Tooth | Trichaptum | biforme | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Giraffe Spots | Peniophora | albobadia | | 1 | Peniophoraceae | Russulales | Agaricomycetes | Fungi |
| False Turkey Tail | Stereum | ostrea | | 1 | Stereaceae | Russulales | Agaricomycetes | Fungi |
| Fan-shaped Jelly Fungus | Dacrymyces | spathularia | | 1 | Dacrymycetaceae | Dacrymycetales | Dacrymycetes | Fungi |
| Honeydew Eater | Scorias | spongiosa | | 2 | Capnodiaceae | Capnodiales | Dothideomycetes | Fungi |
| Black Jelly Drops | Bulgaria | inquinans | | 1 | Phacidiaceae | Phacidiales | Leotiomycetes | Fungi |
| Gabled False Morel | Neogyromitra | brunnea | | 1 | Discinaceae | Pezizales | Pezizomycetes | Fungi |
| Hairy Rubber Cup | Galiella | rufa | | 1 | Sarcosomataceae | Pezizales | Pezizomycetes | Fungi |
| Devil's Urn | Urnula | craterium | | 2 | Sarcosomataceae | Pezizales | Pezizomycetes | Fungi |
| Moth Urchin Fungus | Akanthomyces | aculeatus | | 1 | Cordycipitaceae | Hypocreales | Sordariomycetes | Fungi |
| Trooping Cordyceps | Cordyceps | militaris | | 1 | Cordycipitaceae | Hypocreales | Sordariomycetes | Fungi |
| Witches Butter | Tremella | mesenterica | | 1 | Tremellaceae | Tremellales | Tremellomycetes | Fungi |
| Common Scouring Rush | Equisetum | hyemale ssp. affine | | 1 | Equisetaceae | Equisetales | Equisetopsida | Plantae |
| Flat-branched Tree Clubmoss | Dendrolycopodium | obscurum | | 1 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Southern Ground Cedar | Diphasiastrum | digitatum | | 1 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Shining Clubmoss | Huperzia | lucidula | | 1 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Common Clubmoss | Lycopodium | clavatum | | 1 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Meadow Spikemoss | Lycopodioides | apodum | | 1 | Selaginellaceae | Selaginellales | Lycopodiopsida | Plantae |
| Harbinger of Spring | Erigenia | bulbosa | | 1 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Dwarf Ginseng | Panax | trifolius | | 2 | Araliaceae | Apiales | Magnoliopsida | Plantae |
| Common Jack in the Pulpit | Arisaema | triphyllum | | 1 | Araceae | Arales | Magnoliopsida | Plantae |
| False Solomon's-seal | Maianthemum | racemosum | | 1 | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Smooth Solomon's-seal | Polygonatum | biflorum var. biflorum | | 1 | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Clasping Venus Looking-glass | Triodanis | perfoliata | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Smooth Rockcress | Boechera | laevigata | | 1 | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Deptford Pink | Dianthus | armeria | | 1 | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Wild Pink | Silene | caroliniana ssp. pensylvanica | | 1 | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Virginia Springbeauty | Claytonia | virginica | | 2 | Montiaceae | Caryophyllales | Magnoliopsida | Plantae |
| Jumpseed | Persicaria | virginiana | | 1 | Polygonaceae | Caryophyllales | Magnoliopsida | Plantae |
| American Holly | Ilex | opaca | | 1 | Aquifoliaceae | Celastrales | Magnoliopsida | Plantae |
| Trailing Arbutus | Epigaea | repens | | 3 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Waxflower Shinleaf | Pyrola | elliptica | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Wild Indigo | Baptisia | tinctoria | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Bottle Gentian | Gentiana | clausa | | 1 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Virginia Pennywort | Obolaria | virginica | | 2 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Violet Woodsorrel | Oxalis | violacea | | 1 | Oxalidaceae | Geraniales | Magnoliopsida | Plantae |
| Wild Bergamot | Monarda | fistulosa | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Lyre-leaved Sage | Salvia | lyrata | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Hairy Skullcap | Scutellaria | elliptica var. elliptica | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Beechdrops | Epifagus | virginiana | | 1 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Canadian Lousewort | Pedicularis | canadensis | | 1 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Sessile-leaved Bellwort | Uvularia | sessilifolia | | 1 | Colchicaceae | Liliales | Magnoliopsida | Plantae |
| Wild Yam | Dioscorea | villosa | | 1 | Dioscoreaceae | Liliales | Magnoliopsida | Plantae |
| Indian Cucumber | Medeola | virginiana | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Flypoison | Amianthium | muscitoxicum | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Nodding Trillium | Trillium | cernuum | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Sessile Trillium | Trillium | sessile | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Purple-leaved Willowherb | Epilobium | coloratum | | 1 | Onagraceae | Myrtales | Magnoliopsida | Plantae |
| Showy Orchid | Galearis | spectabilis | | 1 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Squirrel Corn | Dicentra | canadensis | | 1 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Wild Ginger | Asarum | canadense | | 2 | Aristolochiaceae | Piperales | Magnoliopsida | Plantae |
| Woolgrass | Scirpus | cyperinus | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Leafy Bulrush | Scirpus | polyphyllus | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Hedgehog Woodrush | Luzula | echinata | | 2 | Juncaceae | Poales | Magnoliopsida | Plantae |
| Twinleaf | Jeffersonia | diphylla | | 2 | Berberidaceae | Ranunculales | Magnoliopsida | Plantae |
| Round-lobed Hepatica | Anemone | americana | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Allegheny Stonecrop | Hylotelephium | telephioides | | 1 | Crassulaceae | Rosales | Magnoliopsida | Plantae |
| American Golden Saxifrage | Chrysosplenium | americanum | | 1 | Saxifragaceae | Rosales | Magnoliopsida | Plantae |
| Two-leaved Miterwort | Mitella | diphylla | | 2 | Saxifragaceae | Rosales | Magnoliopsida | Plantae |
| Purple Bluet | Houstonia | purpurea var. purpurea | | 1 | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| Wild Blue Phlox | Phlox | divaricata var. divaricata | | 1 | Polemoniaceae | Solanales | Magnoliopsida | Plantae |
| Moss Phlox | Phlox | subulata | | 1 | Polemoniaceae | Solanales | Magnoliopsida | Plantae |
| Narrow Mushroom-Headed Liverwort | Marchantia | quadrata | | 1 | Marchantiaceae | Marchantiales | Marchantiopsida | Plantae |
| Eastern Red Cedar | Juniperus | virginiana var. virginiana | | 1 | Cupressaceae | Pinales | Pinopsida | Plantae |
| Mountain Spleenwort | Asplenium | montanum | | 3 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Lobed Spleenwort | Asplenium | pinnatifidum | | 3 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Blackstem Spleenwort | Asplenium | resiliens | | 1 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Walking Fern | Asplenium | rhizophyllum | | 2 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Maidenhair Spleenwort | Asplenium | trichomanes ssp. trichomanes | | 1 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Eastern Brackenfern | Pteridium | aquilinum var. latiusculum | | 1 | Dennstaedtiaceae | Polypodiales | Polypodiopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |
| Northern Maidenhair Fern | Adiantum | pedatum | | 1 | Pteridaceae | Polypodiales | Polypodiopsida | Plantae |
| Purple Cliffbrake | Pellaea | atropurpurea | | 1 | Pteridaceae | Polypodiales | Polypodiopsida | Plantae |