| Gray Treefrog | Hyla | versicolor | | 1 | Hylidae | Anura | Amphibia | Animalia |
| Spring Peeper | Pseudacris | crucifer crucifer | | 1 | Hylidae | Anura | Amphibia | Animalia |
| American Bullfrog | Lithobates | catesbeianus | | 1 | Ranidae | Anura | Amphibia | Animalia |
| Pickerel Frog | Lithobates | palustris | | 2 | Ranidae | Anura | Amphibia | Animalia |
| Northern Dusky Salamander | Desmognathus | fuscus | | 2 | Plethodontidae | Caudata | Amphibia | Animalia |
| Allegheny Mountain Dusky Salamander | Desmognathus | ochrophaeus | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Spring Salamander | Gyrinophilus | porphyriticus | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Four-toed Salamander | Hemidactylium | scutatum | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Northern Slimy Salamander | Plethodon | glutinosus | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Red Salamander | Pseudotriton | ruber | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Eastern Newt | Notophthalmus | viridescens | | 1 | Salamandridae | Caudata | Amphibia | Animalia |
| Yellow Garden Spider | Argiope | aurantia | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| Eastern Screech-Owl | Megascops | asio | | 1 | Strigidae | Strigiformes | Aves | Animalia |
| No Common Name | Narceus | americanus-annularis | | 1 | Spirobolidae | Spirobolida | Diplopoda | Animalia |
| Leopard Slug | Limax | maximus | | 1 | Limacidae | Stylommatophora | Gastropoda | Animalia |
| Carolina Pine Sawyer | Monochamus | carolinensis | | 1 | Cerambycidae | Coleoptera | Insecta | Animalia |
| Pole Borer | Neandra | brunnea | | 1 | Cerambycidae | Coleoptera | Insecta | Animalia |
| Dogbane Beetle | Chrysochus | auratus | | 2 | Chrysomelidae | Coleoptera | Insecta | Animalia |
| No Common Name | Curculio | sulcatulus | | 1 | Curculionidae | Coleoptera | Insecta | Animalia |
| Canada Thistle Bud Weevil | Larinus | planus | | 1 | Curculionidae | Coleoptera | Insecta | Animalia |
| No Common Name | Efferia | sp. | | 1 | Asilidae | Diptera | Insecta | Animalia |
| Honey Bee | Apis | mellifera | | 1 | Apidae | Hymenoptera | Insecta | Animalia |
| Giant Resin Bee | Megachile | sculpturalis | | 1 | Megachilidae | Hymenoptera | Insecta | Animalia |
| Virgin Tiger Moth | Apantesis | virgo | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Hickory Tussock Moth | Lophocampa | caryae | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| White Spring Moth | Lomographa | vestaliata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Wavy-lined Emerald Moth | Synchlora | aerata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Eastern Tailed-Blue | Cupido | comyntas orientalis | | 1 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Joker Moth | Feralia | jocosa | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Bristly Cutworm Moth | Lacinipolia | renigera | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Monarch | Danaus | plexippus | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Common Buckeye | Junonia | coenia | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Luna Moth | Actias | luna | | 1 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Skimming Bluet | Enallagma | geminatum | | 1 | Coenagrionidae | Odonata | Insecta | Animalia |
| Carolina Grasshopper | Dissosteira | carolina | | 1 | Acrididae | Orthoptera | Insecta | Animalia |
| Differential Grasshopper | Melanoplus | differentialis | | 1 | Acrididae | Orthoptera | Insecta | Animalia |
| Short-winged Meadow Katydid | Conocephalus | brevipennis | | 1 | Tettigoniidae | Orthoptera | Insecta | Animalia |
| Fork-tailed Bush Katydid | Scudderia | furcata | | 1 | Tettigoniidae | Orthoptera | Insecta | Animalia |
| Northern River Otter | Lontra | canadensis | | 1 | Mustelidae | Carnivora | Mammalia | Animalia |
| Ring-necked Snake | Diadophis | punctatus | | 3 | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Ratsnake | Pantherophis | alleghaniensis | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Red-bellied Snake | Storeria | occipitomaculata | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Common Gartersnake | Thamnophis | sirtalis | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Snapping Turtle | Chelydra | serpentina | | 1 | Chelydridae | Testudines | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 1 | Emydidae | Testudines | Reptilia | Animalia |
| Black-staining Polypore | Meripilus | sumstinei | | 1 | Meripilaceae | Polyporales | Agaricomycetes | Fungi |
| Hexagonal-pored Polypore | Neofavolus | alveolaris | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Crown-tipped Coral | Artomyces | pyxidatus | | 1 | Auriscalpiaceae | Russulales | Agaricomycetes | Fungi |
| Devil's Urn | Urnula | craterium | | 1 | Sarcosomataceae | Pezizales | Pezizomycetes | Fungi |
| Cypress Spurge | Euphorbia | cyparissias | | 1 | Euphorbiaceae | Euphorbiales | Magnoliopsida | Plantae |
| Nakedflower Tick-trefoil | Hylodesmum | nudiflorum | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| White Sweetclover | Melilotus | albus | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Spreading Dogbane | Apocynum | androsaemifolium | | 1 | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Eastern Waterleaf | Hydrophyllum | virginianum | | 1 | Boraginaceae | Lamiales | Magnoliopsida | Plantae |
| American Speedwell | Veronica | americana | | 1 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Yellow Corydalis | Corydalis | flavula | | 1 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Dutchman's Breeches | Dicentra | cucullaria | | 1 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Fringed Polygala | Polygaloides | paucifolia | | 1 | Polygalaceae | Polygalales | Magnoliopsida | Plantae |
| Twinleaf | Jeffersonia | diphylla | | 1 | Berberidaceae | Ranunculales | Magnoliopsida | Plantae |
| Purple-flowering Raspberry | Rubus | odoratus | | 1 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Moth Mullein | Verbascum | blattaria | | 1 | Scrophulariaceae | Scrophulariales | Magnoliopsida | Plantae |
| Fall Phlox | Phlox | paniculata | | 1 | Polemoniaceae | Solanales | Magnoliopsida | Plantae |
| Field Pansy | Viola | bicolor | | 1 | Violaceae | Violales | Magnoliopsida | Plantae |
| Canadian White Violet | Viola | canadensis | | 1 | Violaceae | Violales | Magnoliopsida | Plantae |
| Bird's-foot Violet | Viola | pedata | | 1 | Violaceae | Violales | Magnoliopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |